advantage 2 polymerase mix user manual

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advantage 2 polymerase mix user manual

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advantage 2 polymerase mix user manual

Simply reconstitute the mix by adding PCR-grade water along with primers and template. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. The premix contains all neceeary reagents for a high yield and high-fidelity PCR. Please refer to User Manual for the composition. EcoDry Premix are packaged as individual 8-well tube strips with optically clear sealing caps.They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab. Use this guide to prevent common PCR problems. All Rights Reserved. All trademarks are the property of Takara Bio Inc. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com. Through our Takara, Clontech, and Cellartis brands, our mission is to develop high-quality innovative tools and services to accelerate discovery. We have a variety of best-in-class products that can help your AD research move forward. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin.

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Read our cookie policy.With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6kb. The Advantage 2 Polymerase Mix includes two optimized buffers.With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6 kb. The Advantage 2 Polymerase Mix includes two optimized buffers.Advantage 2 Polymerase Mix is highly recommended for applications downstream of our SMART(er) method for cDNA synthesis. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix.They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix.They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. The premix contains all neceeary reagents for a high-yield and high-fidelity PCR. Please refer to User Manual for the composition. EcoDry Premix are packaged as individual 8-well tube strips with optically clear sealing caps.

Please try again or contact Customer Service. Traditional methods of cloning a DNA sequence into a vector and replicating it in a living cell often require days or weeks of work, but amplification of DNA sequences by PCR requires only hours. While most biochemical analyses, including nucleic acid detection with radioisotopes, require the input of significant amounts of biological material, the PCR process requires very little. Thus, PCR can achieve more sensitive detection and higher levels of amplification of specific sequences in less time than previously used methods. These features make the technique extremely useful, not only in basic research, but also in commercial uses, including genetic identity testing, forensics, industrial quality control and in vitro diagnostics. Basic PCR is commonplace in many molecular biology labs where it is used to amplify DNA fragments and detect DNA or RNA sequences within a cell or environment. However, PCR has evolved far beyond simple amplification and detection, and many extensions of the original PCR method have been described. This chapter provides an overview of different types of PCR methods, applications and optimization. Once assembled, the reaction is placed in a thermal cycler, an instrument that subjects the reaction to a series of different temperatures for set amounts of time. This series of temperature and time adjustments is referred to as one cycle of amplification. Each PCR cycle theoretically doubles the amount of targeted sequence (amplicon) in the reaction. Ten cycles theoretically multiply the amplicon by a factor of about one thousand; 20 cycles, by a factor of more than a million in a matter of hours. In the denaturation process, the two intertwined strands of DNA separate from one another, producing the necessary single-stranded DNA template for replication by the thermostable DNA polymerase.

Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion. Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix.With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6kb. The Advantage 2 Polymerase Mix includes two optimized buffers.By continuing to view our website we will assume that you are happy with it. Ok. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above. When you select your country, you agree that we can place these functional cookies on your device. After that, you will need to contact Customer Service to unlock your account. Please try again or contact Customer Service. Please request another reset link. Please try again or contact Customer Service. A verified email address is required to access the full functionality of your Promega.com account. Please try again or contact Customer Service. Please try again or contact Customer Service. Please try again or contact Customer Service. Please check your network settings and try again.

This newly synthesized region then acts as a template for primer extension and synthesis of undesired amplification products.The formation of nonspecific products and primer-dimer can compete for reagent availability with amplification of the desired product. Thus, hot-start PCR can improve the yield of specific PCR products. This omission prevents the polymerase from extending primers until the critical component is added at the higher temperature where primer annealing is more stringent. However, this method is tedious and increases the risk of contamination. A second, less labor-intensive approach involves the reversible inactivation or physical separation of one or more critical components in the reaction. Finally, the DNA polymerase can be maintained in an inactive state through chemical modification (Moretti, T. et al 1998). Activity is restored during initial denaturation, allowing hot-start PCR. While basic PCR works well when smaller fragments are amplified, amplification efficiency (and therefore the yield of amplified fragments) decreases significantly as the amplicon size increases over 5kb. This decrease in yield can be attributed to the accumulation of truncated products, which are not suitable substrates for additional cycles of amplification. These products appear as smeared, as opposed to discrete, bands on a gel. They devised an approach using a mixture of two thermostable polymerases to synthesize longer PCR products. Presumably, when the nonproofreading DNA polymerase (e.g., Taq DNA polymerase) misincorporates a dNTP, subsequent extension of the newly synthesized DNA either proceeds very slowly or stops completely. The proofreading polymerase (e.g., Pfu DNA polymerase or Tli DNA polymerase) serves to remove the misincorporated nucleotide, allowing the DNA polymerases to continue extension of the new strand. In addition, template quality is crucial.

Finally, the synthesis of new DNA begins as the reaction temperature is raised to the optimum for the DNA polymerase.If the temperature during the annealing and extension steps are similar, these two steps can be combined into a single step in which both primer annealing and extension take place.Yet numerous instances exist in which amplification of RNA would be preferred. To apply PCR to the study of RNA, the RNA sample must first be converted to cDNA to provide the necessary DNA template for the thermostable polymerase (Figure 1). This process is called reverse transcription (RT), hence the name RT-PCR. Reverse transcriptases have been adapted for use in a variety of in vitro applications including real-time and endpoint RT-PCR, labeled-cDNA probe generation and cDNA library construction. The ideal reverse transcriptase is robust (highly active under a variety of conditions) and converts all primed RNA within a sample to cDNA, regardless of its abundance, length or secondary structure. Genetic engineering and development of proprietary RT-enhancing buffers have led to the commercial availability of new enzymes that offer superior performance over these naturally occurring RTs. Some thermostable DNA polymerases (e.g., Tth DNA polymerase) possess a reverse transcriptase activity, which can be activated by using manganese instead of magnesium as a cofactor (Myers and Gelfand, 1991). After this initial reverse transcription step to produce the cDNA template, basic PCR is carried out to amplify the target sequence. The efficiency of the first-strand synthesis reaction, which can be related to the RNA quality, also will significantly affect amplification results. At room temperature, PCR primers can anneal to template sequences that are not perfectly complementary. Since thermostable DNA polymerases have activity at these low temperatures (although in most cases the activity is less than 25%) the polymerase can extend misannealed primers.

Because fluorescence of these dyes increases dramatically in the presence of double-stranded DNA, DNA synthesis can be monitored as an increase in fluorescent signal. However, preliminary work often must be done to ensure that the PCR conditions yield only specific product. In subsequent reactions, specific amplification can verified by a melt curve analysis. The product length and sequence affect melting temperature (Tm), so the melt curve is used to characterize amplicon homogeneity. Nonspecific amplification can be identified by broad peaks in the melt curve or peaks with unexpected Tm values. By distinguishing specific and nonspecific amplification products, the melt curve adds a quality control aspect during routine use.These probes also can be used to detect single nucleotide polymorphisms (Lee et al. 1993; Bernard et al. 1998). There are several general categories of real-time PCR probes, including hydrolysis, hairpin and simple hybridization probes. These probes contain a complementary sequence that allows the probe to anneal to the accumulating PCR product, but probes can differ in the number and location of the fluorescent reporters. During the annealing step, the probe hybridizes to the PCR product generated in previous amplification cycles. With this approach, preliminary experiments must be performed prior to the quantitation experiments to show that the signal generated is proportional to the amount of the desired PCR product and that nonspecific amplification does not occur. The hairpin probe is designed so that the probe binds preferentially to the target DNA rather than retains the hairpin structure. As the reaction progresses, increasing amounts of the probe anneal to the accumulating PCR product, and as a result, the fluor and quencher become physically separated. The fluor is no longer quenched, and the level of fluorescence increases.

One advantage of this technique is that hairpin probes are less likely to mismatch than hydrolysis probes (Tyagi et al. 1998). However, preliminary experiments must be performed to show that the signal is specific for the desired PCR product and that nonspecific amplification does not occur. In the first approach, the energy emitted by the fluor on one probe is absorbed by a fluor on the second probe, which hybridizes nearby. In the second approach, the emitted energy is absorbed by a second fluor that is incorporated into the PCR product as part of the primer. Both of these approaches result in increased fluorescence of the energy acceptor and decreased fluorescence of the energy donor. The use of hybridization probes can be simplified even further so that only one labeled probe is required. In this approach, quenching of the fluor by deoxyguanosine is used to bring about a change in fluorescence (Crockett and Wittwer, 2001; Kurata et al. 2001). The labeled probe anneals so that the fluor is in close proximity to G residues within the target sequence, and as probe annealing increases, fluorescence decreases due to deoxyguanosine quenching. With this approach, the location of probe is limited because the probe must hybridize so that the fluorescent dye is very near a G residue. The advantage of simple hybridization probes is their ability to be multiplexed more easily than hydrolysis and hairpin probes through the use of differently colored fluors and probes with different melting temperatures (reviewed in Wittwer et al. 2001). These probes also can be used to detect single nucleotide polymorphisms (Lee et al. 1993; Bernard et al. 1998). There are several general categories of real-time PCR probes, including hydrolysis, hairpin and simple hybridization probes. These probes contain a complementary sequence that allows the probe to anneal to the accumulating PCR product, but probes can differ in the number and location of the fluorescent reporters.

Depurination of the template, which is promoted at elevated temperatures and lower pH, will result in more partial products and decreased overall yield. Additives, such as glycerol and dimethyl sulfoxide (DMSO), also help lower the strand-separation and primer-annealing temperatures, alleviating some of the depurination effects of high temperatures.This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. However, a wide variety of applications, such as determining viral load, measuring responses to therapeutic agents and characterizing gene expression, would be improved by quantitative determination of target abundance. Theoretically, this should be easy to achieve, given the exponential nature of PCR, because a linear relationship exists between the number of amplification cycles and the logarithm of the number of molecules. In practice, however, amplification efficiency is decreased because of contaminants (inhibitors), competitive reactions, substrate exhaustion, polymerase inactivation and target reannealing. As the number of cycles increases, the amplification efficiency decreases, eventually resulting in a plateau effect. This point must be determined empirically for different reactions because of the numerous factors that can affect amplification efficiency. Because the measurement is taken prior to the reaction plateau, quantitative PCR uses fewer amplification cycles than basic PCR. This can cause problems in detecting the final product because there is less product to detect. One such approach includes a second primer pair that is specific for a “housekeeping” gene (i.e., a gene that has constant expression levels among the samples compared) in the reaction (Gaudette and Crain, 1991; Murphy et al. 1990).

Amplification of housekeeping genes verifies that the target nucleic acid and reaction components were of acceptable quality but does not account for differences in amplification efficiencies due to differences in product size or primer annealing efficiency between the internal standard and target being quantified. In competitive PCR, a known amount of a control template is added to the reaction. This template is amplified using the same primer pair as the experimental target molecule but yields a distinguishable product (e.g., different size, restriction digest pattern, etc.). The amounts of control and test product are compared after amplification. While these approaches control for the quality of the target nucleic acid, buffer components and primer annealing efficiencies, they have their own limitations (Siebert and Larrick, 1993; McCulloch et al. 1995), including the fact that many depend on final analysis by electrophoresis.Some of these analyses rely on blotting techniques, which introduce another variable due to nucleic acid transfer efficiencies, while other assays were developed to eliminate the need for gel electrophoresis yet provide the requisite specificity. Real-time PCR, which provides the ability to view the results of each amplification cycle, is a popular way of overcoming the need for analysis by electrophoresis. Specially designed instruments perform both thermal cycling to amplify the target and fluorescence detection to monitor PCR product accumulation. DNA-binding dyes are easy to use but do not differentiate between specific and nonspecific PCR products and are not conducive to multiplex reactions. Fluorescently labeled nucleic acid probes have the advantage that they react with only specific PCR products, but they can be expensive and difficult to design. Some qPCR technologies employ fluorescently labeled PCR primers instead of probes. The dye is simply added to the reaction, and fluorescence is measured at each PCR cycle.

GoTaq qPCR Systems contain BRYT Green Dye, which provides maximum amplification efficiency and greater fluorescence than SYBR Green. Many of these suggestions also apply when using other DNA polymerases. Template DNA concentration, chelating agents present in the sample (e.g., EDTA or citrate), dNTP concentration and the presence of proteins all can affect the amount of free magnesium in the reaction. In the absence of adequate free magnesium, Taq DNA polymerase is inactive. Excess free magnesium reduces enzyme fidelity (Eckert and Kunkel, 1990) and may increase the level of nonspecific amplification (Williams, 1989; Ellsworth et al. 1993). For these reasons, researchers should empirically determine the optimal magnesium concentration for each target. The effect of magnesium concentration and the optimal concentration range can vary with the particular DNA polymerase. Before assembling the reactions, be sure to thaw the magnesium solution completely prior to use and vortex the magnesium solution for several seconds before pipetting. Magnesium chloride solutions can form concentration gradients as a result of multiple freeze-thaw cycles, and vortex mixing is required to obtain a uniform solution. These two steps, though seemingly simple, eliminate the cause of many failed experiments. The free magnesium changes of 0.6mM observed in their experiments dramatically affected amplification yields in an allele-specific manner. They postulated that magnesium chloride precipitates as a result of multiple freeze-thaw cycles. Amplifications were performed using various Mg concentrations to demonstrate the effect on the amplification of a 1.8kb target luciferase gene. The reaction products were analyzed by agarose gel electrophoresis followed by ethidium bromide staining.The buffer regulates the pH of the reaction, which affects DNA polymerase activity and fidelity.

The buffer also contains a compound that increases the density of the sample so that it will sink into the well of the agarose gel, allowing reactions to be directly loaded onto an agarose gel without the need for loading dye. The yellow dye migrates at a rate faster than primers ( 2 at a concentration of 7.5mM for a final concentration of 1.5mM. In most cases, this is an excess of enzyme, and adding more enzyme will not significantly increase product yield.Accurate dispensing of small volumes of enzyme solutions in 50% glycerol is difficult, so we strongly recommend preparing a reaction master mix, which requires a larger volume of each reagent, to reduce pipetting errors. Also, avoid primers with intra- or intermolecular complementary sequences to minimize the production of primer-dimer. Intramolecular regions of secondary structure can interfere with primer annealing to the template and should be avoided. Primers can be designed to include sequences that are useful for downstream applications.Reagents commonly used to purify nucleic acids (salts, guanidine, proteases, organic solvents and SDS) are potent inactivators of DNA polymerases. In some cases, the inhibitor is not introduced into the reaction with the nucleic acid template. A good example of this is an inhibitory substance that can be released from polystyrene or polypropylene upon exposure to ultraviolet light (Pao et al. 1993; Linquist et al. 1998). Failure to amplify the control DNA usually indicates the presence of an inhibitor. Additional steps to clean up the DNA preparation, such as phenol:chloroform extraction or ethanol precipitation, may be necessary. For example, of a 4kb plasmid containing a 1kb target sequence, 25% of the input DNA is the target of interest. Thus, approximately 1,000,000-fold more human genomic DNA is required to maintain the same number of target copies per reaction.

Common mistakes include using too much plasmid DNA, too much PCR product or too little genomic DNA as the template. Reactions with too little DNA template will have low yields, while reactions with too much DNA template can be plagued by nonspecific amplification. When reamplifying a PCR product, the concentration of the specific PCR product is often not known. We recommend diluting the previous amplification reaction 1:10 to 1:10,000 before reamplification. The lengths and temperatures for the other steps of a PCR cycle do not usually vary significantly. However in some cases, the denaturation cycle can be shortened or a lower denaturation temperature used to reduce the number of depurination events, which can lead to mutations in the PCR products. Using an annealing temperature slightly higher than the primer Tm will increase annealing stringency and can minimize nonspecific primer annealing and decrease the amount of undesired products synthesized. Using an annealing temperature lower than the primer Tm can result in higher yields, as the primers anneal more efficiently at the lower temperature. In many cases, nonspecific amplification and primer-dimer formation can be reduced through optimization of annealing temperature, but if undesirable PCR products remain a problem, consider incorporating one of the many hot-start PCR methods. The Tm also can be calculated using the Biomath Calculators. Numerous formulas exist to determine the theoretical Tm of nucleic acids (Baldino, Jr. et al. 1989; Rychlik et al. 1990). The formula below can be used to estimate the melting temperature for oligonucleotides: The risk of undesirable PCR products appearing in the reaction increases as the cycle number increases, so we recommend performing only enough cycles to synthesize the desired amount of product.

If nonspecific amplification products accumulate before sufficient amounts of PCR product can be synthesized, consider diluting the products of the first reaction and performing a second amplification with the same primers or primers that anneal to sequences within the desired PCR product (nested primers). There are many PCR enhancers, which can act through a number of different mechanisms. These reagents will not enhance all PCRs; the beneficial effects are often template- and primer-specific and will need to be determined empirically. Some of the more common enhancing agents are discussed below. GC-rich templates can be problematic due to inefficient separation of the two DNA strands or the tendency for the complementary, GC-rich primers to form intermolecular secondary structures, which will compete with primer annealing to the template. Betaine reduces the amount of energy required to separate DNA strands (Rees et al. 1993). DMSO and formamide are thought to aid amplification in a similar manner by interfering with hydrogen bond formation between two DNA strands (Geiduschek and Herskovits, 1961). Concentrations of DMSO greater than 10% and formamide greater than 5% can inhibit Taq DNA polymerase and presumably other DNA polymerases as well (Varadaraj and Skinner, 1994). These additives can increase DNA polymerase stability and reduce the loss of reagents through adsorption to tube walls. Ammonium ions can make an amplification reaction more tolerant of nonoptimal conditions.Use separate work areas and pipettors for pre- and post-amplification steps. Use positive displacement pipettes or aerosol-resistant tips to reduce cross-contamination during pipetting. Wear gloves, and change them often. PCR reagents can be treated with isopsoralen, a photo-activated, cross-linking reagent that intercalates into double-stranded DNA molecules and forms covalent, interstrand crosslinks, to prevent DNA denaturation and replication.

These inter-strand crosslinks effectively render contaminating DNA unamplifiable. UNG treatment prevents replication of uracil-containing DNA by causing the DNA polymerase to stall at the resulting abasic sites. For UNG to be an effective safeguard against contamination, the products of previous amplifications must be synthesized in the presence of dUTP. This is easily accomplished by substituting dUTP for some or all of the dTTP in the reaction. Nonproofreading polymerases will readily incorporate dUTP into a PCR product, but proofreading polymerases incorporate dUTP much less efficiently (Slupphaug et al. 1993; Greagg et al. 1999; Lasken et al. 1996). Since dUTP incorporation has no noticeable effect on the intensity of ethidium bromide staining or electrophoretic mobility of the PCR product, reactions can be analyzed by standard agarose gel electrophoresis. While both methods are effective (Rys and Persing, 1993), UNG treatment has the advantage that both single-stranded and double-stranded DNA templates will be rendered unamplifiable (Longo et al. 1990). Many of the important parameters discussed there also apply to RT-PCR. For a discussion of reverse transcriptases commonly used for RT-PCR, see the Thermostable Polymerases and Reverse Transcriptases section (below). Procedures for creating and maintaining a ribonuclease-free (RNase-free) environment to minimize RNA degradation are described in Blumberg, 1987. For optimal results, the RNA template, whether a total RNA preparation, an mRNA population or a synthesized RNA transcript, should be DNA-free to avoid amplification of contaminating DNA. The most commonly used DNA polymerases for PCR have no reverse transcriptase activity under standard reaction conditions, and thus, amplification products will be generated only if the template contains trace amounts of DNA with similar sequences. The specific 540bp amplicon is indicated.For example, a primer that anneals specifically to the 3?

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advanta bed user manual

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advanta bed user manual

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advanta bed user manual

Failure to observe this restriction can result in severe injury to people and serious damage to equipment. Chapter 6: General Procedures Cleaning, preventive maintenance, and other general procedures are described in this chapter.A variety of options are available, including nurse call, patient scale, a Patient Position Monitoring (PPM) system, night light, air system, and siderail controls.The automatic contour can only be activated from the patient siderail control panels, and not from the caregiver siderail control panels. The Trendelenburg angle indicator, located on the footboard control panel, displays the amount of Trendelenburg or Reverse Trendelenburg in degrees and inches. The head motor takes 30 seconds to resume its normal operation. To operate the CPR Release: Activate the CPR Release by pushing one of the red switches (A) on the side of the release handle (B), and pulling out on the handle (see figure 1-5 on page. The pedal controls can be accessed near the casters at each of the four corners of the bed. The pedals are clearly identified to distinguish between braking (orange) and steering (green). IV Rod Accommodation There are four locations where an IV rod can be installed—two at the head end, and two at the foot end. To store a siderail, lower it completely, then push it in under the bed surface. Always tug slightly on a siderail after adjusting it to ensure that it is securely locked in place. Press and release the appropriate heel relief switch (heel 1, heel 2, or heel 3). No additional electrical components, such as isolation transformers, are required to make this bed meet applicable electrical codes. The insulation materials are of the type that will not deteriorate to an unsafe level through normal use. They stop the motor automatically if it heats up to a certain temperature. The motor will not run again until the motor cools.

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Sign in Forgot Password. My Bench Close Sign In Not A Member. Sign Up Join MedWrench OK name type Receive Summary Emails. Designed for comfort. Hill-Rom’s Advanta bed is engineered to help reduce the likelihood of patient falls and offers the added benefit of an optional four-zone pressure reduction surface. While the Advanta bed was created to help address your patients’ needs, it also offers features that contribute to the safety and efficiency of caregivers. FORUMS View All (33) Ask a New Question 2 Replies -DannyFlVic a year ago a year ago Bed head section will not lower Having issues w bed not lowering head side beyond a certain point. Is there a technician in the Daytona Beach, FL area. Reply 1 Reply -frank81 3 years ago 3 years ago service mamual need a service manual Reply 1 Reply -Wiltdflower 3 years ago 3 years ago Bed wont go sown My sis was playing with buttons on bed. Now the bed will not go straight down. The bed goes in trendelenburg if you press the bed down button or will go And reverse trendelenburg if you press the bed up button.The bed is all the way up. It was working fine before.By continuing to browse the site you are agreeing to our use of cookies. Please review our Privacy Policy for more details. All Rights Reserved. P1600 For Parts or Technical Assistance man168ra USA (800) 445-3720 Canada (800) 267-2337 International: Contact your distributor. The information contained in this manual is subject to change without notice. Hill-Rom makes no commitment to update or keep current, the information contained in this manual. The only product warranty intended by Hill-Rom is the express, written warranty accompanying the bill of sale to the original purchaser. To order additional copies of this service manual, call (800) 445-3720, and place a parts order for part number man168ra. It also includes parts lists (in chapter 5) for ordering replacement components. Audience This manual is intended for use by only facility-authorized personnel.

CAUTION: When handling electronic components, wear an antistatic strap. The fittings are plastic and can break if handled too roughly during the removal process. CAUTION: Failure to align the slide switch and drive screw when installing the hilow head drive screw can cause severe damage to the bed. If the hose becomes unattached during operation, equipment damage could occur. CAUTION: Align the slide switch and the head limit switch after servicing the head drive screw assembly. Failure to do so could result in personal injury or equipment damage. A common digital VOM is shown (see figure 2-1 on page 2-4).Note symptoms or other information concerning the problem that the operator describes. This information helps identify the probable cause. 1. The mechanical parts are operating correctly. Remove, replace, and adjust (if necessary) the damaged component(s).The resistance should be above 100K ohms. 3. Apply pressure to the PPM sensor, the resistance should go down as more pressure is applied. This only tests for power being switched to each valve or pump component to determine if a possible fuse has failed or other power failure. The circuits that fail will flash their corresponding LED for 7 seconds. Table 2-3. Air System Self-Diagnostic Test—Surface Power LED and Corresponding LED If Surface Power LED indicator is FLASHING: Corresponding circuit that has Lights this LED indicator on the FAILED footboard control panel. Otherwise, call Hill-Rom Technical Support at (800) 445-3720.Go to “Final Actions” on page 2-17.Replace or repair the damaged pins. Connect the connector. Otherwise, call Hill-Rom Technical Support at (800) 445-3720. 11. Go to “Final Actions” on page 2-17.CAUTION: To prevent component damage, ensure that your hands are clean, and handle the P.C. The voltage is greater than 8V DC. Otherwise, call Hill-Rom Technical Support at (800) 445-3720. 9. Go to “Final Actions” on page 2-17.

See table 1-3 on page 1-25 through table 1-5 on page 1-27 for caregiver and patient control switches for the siderails, the caregiver control switches for the footboard control panel, and a list of whether the item is standard or optional. The back light can be turned on or off at the footboard control panel. The back light on the nurse call switches cannot be turned off. The head, knee, and hilow motors can be locked out in any combination, or all at once. Activation of the knee lockout also keeps the automatic contour function from working. To place a call on the nurse call system, press the nurse switch on any siderail control panel. A tone sounds to confirm that the call has been placed, and the yellow call indicator light on the patient’s siderail control panel illuminates. For A model beds: Turn on the system by simultaneously pressing and holding the enable switch and the mode switch. Hold any two volume switches, then press and release the third volume switch to change the tone. All the PPM indicator lights will begin to flash. Failure to do so could result in personal injury or equipment damage. SHOCK HAZARD: Do not expose the bed to excessive moisture. Personal injury or equipment damage could occur. SHOCK HAZARD: If fluid is spilled in the footboard area, unplug the bed from its power source. Preventive maintenance performed by unauthorized personnel could result in personal injury or equipment damage. WARNING: Tie the foot section back toward the head section when preparing to remove the foot end cover. Failure to disconnect line voltage to the bed can cause personal injury and damage the VOM. WARNING: Use a 2 x 4 to support the head end of the bed during this procedure. Failure to do so could cause the head end of the bed to fall resulting in person al injury or equipment damage. Operate the bed only with persons clear of mechanisms. CAUTION: To prevent component damage, ensure that your hands are clean and only handle the P.C. boards by their edges.

board and the logic control P.C. board. This cable, with its multi-colored wires, is an exception to the single color rule. This interface is earth-ground-referenced. An AC line supply connector, P5, is provided for accessories or air system control P.C. The power supply provides a pre-regulated 12-14V DC referenced to signal VSS at 400mA maximum, 200mA typical, and another unregulated 9-18V DC referenced to signal chassis digital ground at 200mA. The analog circuitry is located in the weigh frame of the bed as close to the load beams as possible. The load beams are resistive bridge sensors excited by a regulated DC voltage. The relay is on continuously during normal operation. U15 can maintain regulation with an input voltage down to 5.75V DC. R46 scales the range of R63 pin 16 to the 2.90 volts described above. The remainder of the conversion instruction is sent by clocking in three more bits, 101, where 101 is the opcode for converting U4 CH 1 with respect to CH 0. Communications between the controller circuit and the pressure sensing circuit is via a 25 pin D-sub cable, utilizing 3-wire, synchronous, serial, bi-directional communications (along with other discrete lines, and AC and DC power). Pressure Relief Mode While in the pressure relief mode, the air pressures in each of the six bladder zones are regulated at predefined levels, called trim points. Auto Firm Mode While in the auto firm mode, all trim points are changed to new values. Pressures are then regulated, as described in the pressure relief mode, at the new trim points. However, the head and seat zones are inflated to these new values first. These failures cause the system to transfer to the off mode and repeatedly flash a code on the footboard control panel service required LED. Thoroughly dry the electrical connections for the footboard before plugging the bed into its power source. Personal injury or equipment damage could occur. Replacement 1. Install the footboard (A) on the mounting posts (B).

CAUTION: To prevent component damage, ensure that your hands are clean, and handle the P.C. The voltage is greater than 100V AC. Perform the “Air System Self-Diagnostic Test”. Go to “Final Actions” on page 2-17. Failure to do so could result in personal injury or equipment damage. Otherwise, call Hill-Rom Technical Support at (800) 445- 3720. 7. Replace the air control P.C. board (refer to procedure 4.9). CAUTION: Wear an appropriate static strap when working with electronics. Equipment damage can occur. CAUTION: To prevent component damage, ensure that your hands are clean, and handle the P.C. If this solves the problem, go to “Final Actions” on page 2-17. Otherwise, call Hill-Rom Technical Support at (800) 445-3720. 5. Go to “Err0” on page 2-61, “Err1” on page 2-62, “Err2” on page 2-62, or “Err3”. An error has occurred during the memory check. If the Position LED is flashing, proceed to step 1. Exiting LED 14. The Exiting LED is flashing. See “Zeroing for the Out of bed Mode (Beds Without Scales) for beds without scales on page 1-34, or see “Zeroing the Scale”. Page 2 - 68. The Nurse Call light on the tester comes on when either switch is activated. NOTE: Pressing the Nurse Call switch from the siderail causes the call to be placed and then released, and is controlled by the bed system. Figure 3-11. Logic Board PN 63285 Refer to fold-out FO 3-7 at the rear of this manual. Figure 3-12. Figure 3-16. Sensor Board PN 4474001 Refer to fold-out FO 3-12 at the rear of this manual. Figure 3-17. Figure 3-21. Inner Patient II Board (B Model Beds) PN 67203 Refer to fold-out FO 3-17 at the rear of this manual. Figure 3-22. The outputs on connectors P7 and P12 are switched AC line voltages used to power the split-phase, AC, intermittent-duty motors. All inputs to the logic control P.C. board are processed simultaneously and present the motors with power when conditions are correct. The siderail cable, part number 63600, connects the siderail interface P.C.

If the bed is unloaded too far, the hilow remote switch trips and the bed automatically rises for three seconds. Replacement 1. Install the brake light switch in the reverse order of removal. 2. Replacement 1. Replacement Install the siderails in the reverse order of removal. See “Scale Calibration” on page 6-3. See “Lubrication Requirements” on page 6-7. 5. If the siderails are frozen: a. Raise the siderail to the up position, or remove it from the bed completely. SHOCK HAZARD: Unplug the bed from its power source. CAUTION: To prevent component damage, ensure that your hands are clean and only handle the P.C. boards by their edges. CAUTION: When handling electronic components, wear an antistatic strap. SHOCK HAZARD: Unplug the bed from its power source. CAUTION: To prevent component damage, ensure that your hands are clean and only handle the P.C. SHOCK HAZARD: Unplug the bed from its power source. Failure to do so could result in personal injury or equipment damage. CAUTION: To prevent component damage, ensure that your hands are clean and only handle the P.C. SHOCK HAZARD: Unplug the bed from its power source. Failure to do so could result in personal injury or equipment damage. CAUTION: To prevent component damage, ensure that your hands are clean and only handle the P.C. Seat Section Panel Removal 8. Using the needle nose pliers, push the four drive rivets (C) securing the PPM sensors (D) to the bed, through the bed frame and PPM sensor (D). 9. PPM Sensor Removal NOTE: Do not remove the cable ties holding the cables in the electronics pan if possible. It is preferred to feed the sensor cable through the cable ties. 13. CAUTION: Ensure there are no sharp edges on the drive rivets. Equipment damage can occur. SHOCK HAZARD: Unplug the bed from its power source. If requested by Hill-Rom, products or parts for which a warranty claim is made shall be returned prepaid to Hill-Rom’s factory. This packet includes an RMA number, instructions, and a shipping label.

If an RMA number is not available, obtain one by phoning Hill-Rom Technical Support at (800) 445-3720. Page 5 - 6. Do not confuse this price with your price. Do not return any parts without an RMA number.Air Supply Module Item Number Part Number Quantity Description 39763-04 (1600) 2 Standoff 987 (1600) Machine Screw 63909 (1600) Air system weld - assembly 47103 (1600) Muffler assembly 64095 (1600) Linear air pump 64096 (1600) Elbow fitting 19124 (1600) Footboard—P1606 Item Number Part Number Quantity Description 6371903 (1600) Cable assembly (black) 6371902 (1600) Cable assembly (red) 6371901 (1600) Cable assembly (white) 63344 (1600) 0 or 1 Scale display assembly (beds with scales) 6371904 (1600) Cable assembly (blue) 4214101 (1600) Head Drive Screw Assembly Item Number Part Number Quantity Description 43272S (1600) Head drive screw assembly 3517 (1600) Roll pin 42141 (1600) Hilow screw 66266S (1600) Clip engagement 61991 (1600) Slide switch assembly 42845 (1600) Lift nut. Electronics Module Assembly Item Number Part Number Quantity Description 45916 (1600) Test port lid 45915 (1600) Test port box 63208 (1600) P.C. board assembly, inner patient switch 63600 (1600) Siderail interface cable (A model beds) 6010802 (1600) Cable 64027 (1600) The software does not allow the user to key in weights outside of this range. NOTE: When the display reads CAL1, the bed is zeroed. 8. When one tone sounds, add the calibration weight to the left foot section. 9. Cleaning SHOCK HAZARD: Follow the product manufacturer’s instructions. Failure to do so could result in personal injury or equipment damage. SHOCK HAZARD: Unplug the unit from its power source. Failure to do so could result in personal injury or equipment damage. To loosen heavy, dried-on soil, you may first need to saturate the spot. Disinfecting When there is visible soilage and between patients, we recommend that you disinfect the unit with a tuberculocidal disinfectant.

NOTE: The footboard connectors are keyed so that footboards will fit easily on the correct beds. SHOCK HAZARD: Unplug the bed from its power source. Failure to do so could result in personal injury or equipment damage. Replacement CAUTION: Do not lower the foot section while the knee section is down and the foot end cover is removed. Failure to do so could result in personal injury or equipment damage. 5. Install the footboard (see “Footboard” on page 4-7) on the bed. 6. Failure to do so could result in personal injury or equipment damage. 2. Unplug the bed from its power source. 3. SHOCK HAZARD: Unplug the bed from its power source. Damage can occur to the pressure switches. Failure to do so could result in personal injury or equipment damage. 1. Failure to do so could result in personal injury or equipment damage. CAUTION: When handling electronic components, wear an antistatic strap. Failure to do so, could result in component damage. Failure to do so could result in personal injury or equipment damage. NOTE: Ensure that the air tubes are connected to their corresponding valves. 2. SHOCK HAZARD: Unplug the bed from its power source. CAUTION: When handling electronic components, wear an antistatic strap. Failure to do so, could result in component damage. SHOCK HAZARD: Unplug the bed from its power source. Failure to do so could result in personal injury or equipment damage. Replacement 1. SHOCK HAZARD: Unplug the bed from its power source. SHOCK HAZARD: Unplug the bed from its power source. Failure to do so could result in equipment damage. CAUTION: Be careful not to damage the cables when removing the footboard control panel. Detach the lower end of the cable conduit from the retracting frame. 13. Cut the wire ties. 14. Remove the screw and clamp attaching the power cable (F) to the bed frame. SHOCK HAZARD: Unplug the bed from its power source. Failure to do so could result in personal injury or equipment damage.

SHOCK HAZARD: Unplug the bed from its power source. Failure to do so could result in personal injury or equipment damage. SHOCK HAZARD: Unplug the bed from its power source. Ensure that the CPR latch assembly properly actuates the limit switch. Damage to the bed can occur. 10. Failure to do so could cause the head end of the bed to fall, resulting in personal injury or equipment damage. If the bed is unloaded too far, the hilow remote switch trips and the bed automatically rises for three seconds. Remove the hilow head drive screw assembly (J) from the bed. Replacement 1. Install the hilow head drive screw assembly (J) on the bed, making sure that the screw (I) goes into the keyhole slot. CAUTION: Do not over-tighten the limit switch mounting screws. Over-tightening the mounting screws could cause the limit switch to malfunction. 2. Over-tightening the mounting screws can cause the limit switch to malfunction. 3. Failure to do so could cause the head end of the bed to fall, resulting in personal injury or equipment damage. If the bed is unloaded too far, the hilow remote switch trips and the bed automatically rises for three seconds. Failure to do so could result in personal injury or equipment damage. 4. Unplug the bed from its power source. 5. SHOCK HAZARD: Unplug the bed from its power source. Failure to do so could cause the foot end of the bed to fall, resulting in person al injury or equipment damage. If the bed is unloaded too far, the hilow remote switch trips and the bed automatically rises for three seconds. Using the screwdriver if necessary, remove the hilow straps (O) from the torque cage (P). 16. Slide the hilow foot drive screw assembly toward the head end of the bed until the screw (I) can be lifted from the keyhole slot. Install the seat section cover (see “Air Control Cover” on page 4-15). 13. Install the foot end cover (see “Foot End Cover” on page 4-9). 14. Plug the bed into an appropriate power source. 15.

Preventive maintenance performed by unauthorized personnel could result in personal injury or equipment damage.Knee limits Run the knee section to the full upper and lower limits to ensure proper function of the limit switches. Pivot points Lubricate all pivot points on the bed. CPR release Test the CPR release for proper operation and reset of the head screw drive. Adjust the CPR release if necessary (see “CPR Release Handle”. We recommend that you perform annual preventive maintenance (PM) and testing for Joint Commission on Accreditation of Healthcare Organizations (JCAHO). Check the switch LEDs and the LED information indicators for proper operation. Failure to do so could result in personal injury or equipment damage. 1. Unplug the bed from its power source. 2. Lift the foot section (B) to access the air hose coupling (D) and cable connector (C) (see figure 7-1 on page 7-4). Failure to do so could result in personal injury or equipment damage. 1. Comfortline Basic is a registered trademark of Hill-Rom Services, Inc. Comfortline Ultimate is a registered trademark of Hill-Rom Services, Inc. Hill-Rom is a registered trademark of Hill-Rom Services, Inc. PLEUR-EVAC is a registered trademark of Deknatel, Inc. SideCom is a registered trademark of Hill-Rom Services, Inc. ZoneAire is a registered trademark of Hill-Rom Services, Inc. The information contained in this manual is subject to change without notice. The only product warranty intended by Hill-Rom is the express, written warranty accompanying the bill of sale to the original purchaser. Hill-Rom makes no other warranty, express or implied, and in particular, makes no warranty of merchantability or fitness for a particular purpose. Additional copies of this manual can be obtained from Hill-Rom. To order additional copies of this manual, call (800) and place a parts order for part number usr017rb.

Revision Letter Pages Affected Date Original Issue December, 1999 A All April, 2001 B All September, 2002 Note the following examples: Standard text used for regular information. Boldface text emphasizes a word or phrase.The symbol below highlights a WARNING or Warning and Caution A WARNING identifies situations or actions that may affect patient or user safety. Disregarding a warning could result in patient or user injury. A CAUTION points out special procedures or precautions that personnel must follow to avoid equipment damage. The symbol below highlights a CAUGHT HAZARD WARNING: Caught Hazard Warning The symbol below highlights a CHEMICAL HAZARD WARNING: Chemical Hazard Warning The symbol below highlights an ELECTRICAL SHOCK HAZARD WARNING: Electrical Shock Hazard Warning 1 Accuracy of the scale may be diminished if the patient weighs more than 400 lb (181 kg). Mattress interface pressure performance may be diminished if the patient weighs more than 300 lb (136 kg). WARNING: Do not use the product outside the recommended patient height, width, and weight ranges. Patient injury or equipment damage may occur. 3 The following tables list the caregiver controls for the siderails and footboard, and the patient controls for the siderails. The tables also indicate whether the item is standard or optional. The patient siderail controls are on the inner sides of the head end siderails. 4 When activated, the CPR Release automatically lowers the head section of the bed to the flat position, enabling emergency procedures to be performed without unnecessary loss of time. After the head section is down, the knee section also comes down to the flat position. On beds equipped with an integrated air surface (ZoneAire Sleep Surface), the Auto Firm function engages automatically when the CPR Release is activated. This provides a firm surface that supports a CPR board. The headboard of the Advanta Bed can be used as a CPR board in emergencies.

Emergency CPR Release Button Location: On both sides of the head section, near the head end of the bed. To Activate: Press the red button on either side of the CPR Release handle, and pull the handle. Release your hand as the head section begins to come down. WARNING: If electrical power is lost, the head section still lowers, but the Auto Firm function does not engage. The optional air mattress maintains the same air pressure it had before power loss. The effectiveness of the CPR board may be reduced. Emergency CPR Release Handle If electrical power is lost, the air mattress maintains the same level of pressure it had at the time of power loss. If electrical power is lost, the knee section, if raised, cannot be lowered. Do not use the CPR Release to lower the head section in non-emergency situations. Use the Head Down controls located on the siderail. After activating the CPR Release, allow 30 seconds for the head motor to resume its normal operating position before using the Head Up or Head Down controls. 6 WARNING: Unplug the bed from its power source before inserting the IV rod for manual operation. Unexpected resumption of power can cause the IV rod to rotate and cause injury. Location: Access hole at the head end of bed. To Reset: Insert a Hill-Rom P2217 IV rod into the access hole at the head end of bed until it engages the roll pin in the end of the screw assembly. Push the IV rod hard enough to push the ejection spring back from the roll pin, allowing the IV rod to engage. Turn the IV rod clockwise until the screw assembly reaches its mechanical stop (you should hear a click). The head section will not raise during this procedure. IV Pole Insertion Points for Manual Operation WARNING: Remove the IV rod before plugging the bed into an appropriate power source. Resumption of power can cause the IV rod to rotate and cause injury. Remove the IV rod from the head screw assembly. Plug the bed into its appropriate power source.

Hold the CPR release in the activated position until the motor stops running. The bed is now operational. Central Brake and Steer The Advanta Bed is equipped with 4-wheel braking and 2-wheel steering. The pedal controls can be accessed near the casters at each of the four corners of the bed. Steering the Advanta Bed 7 When the brakes are not engaged, the casters rotate, and the Brake Not Set indicators flash continuously. Location: At each caster on the bed. To Activate: At any wheel, push down fully on the orange pedal (the one closest to the head end of the bed). When the Brake Not Set indicator goes out, the brakes are locked. Unless the bed is about to be moved, the brakes should always be set and locked. After setting the brakes, try to move the bed slightly to ensure that the brakes are locked. When the brakes are not locked, the Brake Not Set indicators flash continuously. Central Brake and Steer Braking Steering The 2-wheel steering system locks both foot end casters in a straight position for easier transport. To Activate: At any wheel, push down fully on the green pedal (the one closest to the foot end of the bed). The Brake Not Set indicators will start to flash. Central Brake and Steer Steering During patient transport and in steer mode, push the Advanta Bed from the head end, using the headboard handles as hand holds. 8 This function is used for exact positioning of the bed and maneuvering in confined areas Location: At each caster on the bed. To Activate: At any wheel, push either the orange or green pedal down slightly until the pedal is flat (in a horizontal position). Central Brake and Steer Neutral Bed Functions The Advanta Bed is equipped with standard bed function controls on the caregiver side of each head end siderail, and at the footboard control panel. These controls operate all of the motor functions of the bed. Location: Outside left siderail, outside right siderail, footboard control panel.

To Activate: Press and hold the Bed Up key to raise the bed. Press and hold the Bed Down key to lower the bed. WARNING: Leave the bed in the low position when the patient is unattended. This can reduce the possibility of patient falls and the severity of any resultant injuries. The Bed Not Down indicator illuminates when the bed is not in the lowest position. 9. The head section of the bed can be raised to any desired angle of inclination up to 65. Using the head angle indicator (see Head Angle Indicator on page 27), you can raise or lower the head section to specific desired angles. Location: Outside left siderail, outside right siderail; left patient siderail, right patient siderail. To Activate: Press and hold the Head Up key to raise the head section of the bed. Press and hold the Head Down key to lower the head section of the bed. Advanta Bed with Head Section Raised Head Angle Indicators, located on the caregiver sides of the head end siderails, display the amount of head section angle in degrees. For the caregiver s convenience, the automatic contour function is only activated from the patient siderail controls. When the Head Up control is activated from the patient siderail controls, the automatic contour function is activated. This raises the knee section automatically up to 15 when the Head Up function is activated. The automatic contour function helps keep the patient from sliding towards the foot end of the bed. This function is not activated from the caregiver controls, but is accessible to caregivers by using the patient siderail controls. Alternatively, the caregiver can simultaneously press the head up and knee up controls. Do not manually lift or lower the head section with the CPR release handle. If you do, the head section will not elevate properly until it is reset. Damage to the bed can occur when the head section is lifted with the CPR release handle. To Activate: Press and hold the Knee Up key to raise the knee section of the bed.

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DOWNLOAD: NAVY ADVANCEMENT MANUAL 2 3 navy advancement manual Review and download this entire user guide or troubleshooting section navy advancement manual, to offers the answer and then for any potential benefit. US NAVY SHIPBOARD PEST CONTROL MANUAL Format: PDF 870 View Last update: 2016-01-27 NAVY STANDARD OPERATING PROCEDURES MANUAL Format: PDF 428 View Last update: 2016-02-04 NAVY OWNERS AND OPERATORS MANUAL 2011 Format: PDF 338 View Last update: 2016-02-08 NAVY MARINE CORPS CORRESPONDENCE MANUAL Format: PDF 429 View Last update: 2016-01-24 4 5 navy advancement manual LEARN AND DOWNLOAD TO OBTAIN SUPPORT MANUFACTURER NAVY ADVANCEMENT MANUAL PROVIDE IT WITH ANY SIGNIFICANT INSIGHTS OR TIPS.We are a non-profit group that run this service to share documents. We need your help to maintenance and improve this website. It looks like your browser needs updating. For the best experience on Quizlet, please update your browser. Learn More. Removed the requirement to not use names (no names used) during post-slating briefs for E-7 to E-9 boards. What is slating? When the panel arranges all the candidates from top to bottom once the review of an entire rating is done. What is the purpose of the Advancement Manual. Provide procedures for administering the advancement in rate system for enlisted members serving active, reserve and inactive reserve in the USN. What previous INST(s) did BUPERSINST 1430.16F cancel? BUPERSINST 1430.16E; BUPERSINST 1430.17F; BUPERSINST 1401.2 Who has the SOLE authority for advancement and SOLE authority for the removal of enlisted personnel selected for advancement by to E7-E9 from a selection board list. Chief of Naval Personnel CHNAVPERS After an E2-E6 met all the criteria of the Navy Enlisted Advancement System (NEAS) who has the independent authority to advance E4-E6. CO's and or a designated OIC What are the significant changes in chpt 1 of BUPERSINST 1430.16F?

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Our payment security system encrypts your information during transmission. We don’t share your credit card details with third-party sellers, and we don’t sell your information to others. Please try again.Please try again.Please try again. It provides instructions for determining eligibility requirements for advancement, preparing necessary forms and messages and effecting advancements and changes in rating. It also contains procedures for ordering, maintaining, and disposing Navy-wide examinations for advancement. Then you can start reading Kindle books on your smartphone, tablet, or computer - no Kindle device required. Register a free business account To calculate the overall star rating and percentage breakdown by star, we don’t use a simple average. Instead, our system considers things like how recent a review is and if the reviewer bought the item on Amazon. It also analyzes reviews to verify trustworthiness. Because of this navy advancement manual guides are far superior as opposed to pdf guid.No longer all course guides are created equal. Some Navy advancement manual required the user to manually install this system to the appropriate file to ensure that the help guide be employed in the program, further, some guides needed an add-on for any gps positioning tool that is a must for correct movement to a higher goal. The next matter an end user will want to look for is the place where many goals the guide will demonstrate at any given time and whether or not you will find further steps needed by the user to create the guide up once placed in the course. Some Navy advancement manual have to be set with what character an individual is applying as a way to set goals, other guides will detect which character has used and automatically create the correct goals for that character. Gold, and finding of artifacts and guide one stage further.

Who is the sole authority for the determination of elgibility of an applicant for a special enlisted selection board, and is the sole authority for approving the report of a special enlisted selection board convened under this inst. CHNAVPERS When does eligibility for the special enlisted selection board terminates? 9 months after the regular board is convened. Whose responsibility is it in ensuring the member's name appears on the selection board eligibility list. The member shares equal responsibility with their parent commands. After exhausting local resources, and the member's name still does not appear on the eligibility list contact NAPERSCOM PERS-811 for enlisted and PERS 812 for reserves. How long does the applicant have to submit the request for a special enlisted selection board. No later than 9 months after the regular board has convened. What information must be incuded in the special enlisted selection board request. What date will be the date for advancement for candidates selected for advancement by a special board. The same date of advancement if they would've been selected at the regular board. Where can you find a definition for the terms used in the advancement manual. Terms can be found in Appendix A Which enlisted personnel does the manual apply to. Personnel on the Regular Navy and Navy Reserve on Active Duty. Personnel of Navy Reserve on Inactive Duty What personnel is considered active duty? (1) Regular Navy and General Assignment Recall (USNR) Active. (2) Full Time Support (FTS). (3) Canvasser Recruiter (CANREC) personnel recalled with obligated service for 1 year or more. Less than 31-day break between periods of active duty constitutes continuous active service. Such personnel will participate in the active duty examination process. (4) Fleet Reserve and retired enlisted personnel recalled to active duty with the Regular Navy. (5) Temporarily commissioned Limited Duty Officers (LDOs), U.S.

Incorporated Special Selection Board guidelines from the cancelled BUPERSINST 1401.2 and I.D additional offices with NEAS-related responsibilities. What is chpt 1 of BUPERSINST 1430.16F? NEAS General Informaton What is the purpose for the Advancement Manual. Determing advancement elgibility reqs, prepping necessary forms, ordering, maintaining custody and disposing of Nav-wide exams, administering exams, changing rating, responsibilities and procedures relating to advancement. The advancement manual applies to the advancement for which enlisted personnel. USNR, FTS, CANREC, Temp Commissione LIMDU, Active duty whose permaent status is enlisted. Reserve on inactive duty who are assigned to a Navy Reserve unit with a drilling req, recalled for active duty for special work (ADSW), 1yr recall, recalled under Presidential Recall Authority or Mobilization regardless of the cumulative amout of time served on active duty. Knowledge, performance, and experience factor. Considers the whole person in its selection criteria. What is the FMS comprised of for E7. Exam score and evals. What is the FMS comprised of for E4-E6. Advancement-in-rate exam score, evals, service in paygrade, awards and previous exam performances. Whats the max PMA? 4.0 For inactive reserves what does TIR equal. Annually by CHNAVPERS How are E8-9 candidates designated SBE (Selection Board Eligible). What are special selection boards. Special enlisted board that considers individuals who were not properly considered through the normal selection board process due to circumstances beyond their control. When are special selection boards held. Annually, or as required to consider any member who was elgible but not properly considered by the last regular board. Those members will be compared to their competitive group from the last regular board.

What authority is to be contacted if member's name still does not appear after all efforts are exhausted. Who makes the final eligibility decision on special selection board requests. CHNAVPERS Which records will the applicant's be compared to. Those in the previously held regular board. If selected, what will be the date of the candidate's advancements. The same date as the candidates who advanced in the previous board. What is the E6 requirement for recommendation for LDO. E6 personnel must pass the E7 advancement-in-rate examination prior to submitting a package for the LDO selection board. Which instruction provides info for enlisted personnel transition to officer status. OPNAVINST 1420.1A What are the CO responsibility in adherence to the advancement manual regulations? (1) To properly administer advancement examinations to recommended, eligible, and fully qualified candidates. (2) To properly maintain the security of examination materials. (3) To obtain and distribute the Bibliography for Advancement-in-Rate Exam Study Guide (BIB). BIB's may be downloaded from (4) To ensure dates of examinations, deadline for completion of requirements, and the requirement for signing worksheets are disseminated throughout the command. (5) To designate a local educational services officer (ESO) in writing to act as liaison between the local ESO and ESO agreeing to administer an examination. (6) To forward examinations for those candidates who will be on leave, Temporary Additional Duty (TEMADD), or in a transient status because of permanent changes of station (PCS) on regularly scheduled examination date. What are Naval Personnel Development Command (NPDC) Responsibilities? (1) To coordinate with COMUSFLTFORCOM and applicable Type Commanders (TYCOMs) to designate the best-qualified rating SMEs (usually 6) covering a wide diversity of experience in the rating for participation in the rating AEDCs.

Navy on active duty, whose permanent status is enlisted. What is the process for advancement from E2 through E3. Automatic advancement for personnel meeting minimum time-in-rate (TIR) requirements. No exam or evaluation required. What are the requirements for advancement to E4 through E7. Examinations are required. What comprises the FMS for E7. Examination score and Performance Evaluations. What comprises the FMS for E4-E6. Advancement-in-rate examination score, performance evaluations, service in paygrade, awards, previous examination performances. What is the maximum PMA points for an advancement candidate. PS: Find the definition for the terms: SIPG and DSPG. What does advancement from paygrades E7-E9 require. Advancement to E7, E8, and E9 requires selection board action. How are E7-E9 candidates qualified for selection board. E7 candidates must be designated selection board eligible (SBE) by competing in a Navy-wide advancement examination and meeting final multiple requirements for their rate. How are E8-E9 candidates designated SBE. Selection boards are convened annually by Chief of Naval Personnel (CHNAVPERS). Which personnel is eligible to special selection boards. Individuals who were not properly considered through the normal selection board process due to circumstances beyond their control. Held annually, or as required, to consider any member who was eligible but not properly considered by the last regular board. Who do special selection boards candidates compete against. Those members will be compared to their competitive group from the last regular selection board. What authority determines eligibility for a special selection board. CHNAVPERS Sole authority for approving the report of a special enlisted selection board. When does the special selection board eligibility terminate? 9 months after the regular board convenes. Who is responsible to ensure the candidate's name appears in the selection board eligibility list.

If you forget it there is no way for StudyStackYou would need to create a new account. It is only used to allow you to reset your password. You may alsoYes, anything under to missing questions it still valid. If it is listed in the Standard Navy Distribution List (SNDL) General Ratings Individual command Personnel meeting minimum time-in-rate (TIR) requirements will be advanced automatically from E2 and E-3 without local action OPNAVINST 1420.1 and NAVPERS 15627 Commanding officer after all advancement requirements are met. Occupational fields The internet Service Record page 13 Close of business the day after administration of each examination Which of the following correctly labels the colors with their respective classifications. SECRET - RED, CONFIDENTIAL - BLUE, and FOR OFFICIAL USE ONLY (FOUO) - BLACK. For a better experience, we recommend using another browser. Learn more Facebook Email or phone Password Forgotten account. Sign Up See more of MyNavy HR on Facebook Log In or Create New Account See more of MyNavy HR on Facebook Log In Forgotten account.Below are some reasons you should consider using t.Have you used the GTCC in the past. Pilot and Hispanic HeroAny HYT waiver must be approved prior to 1 August 2020. Candidates who have submitted a formal written request for voluntary transfer to the retired reserve (with or without pay) or Fleet Reserve on a date prior to their mandated HYT are ineligible for advancement. e. Candidates in a rating requiring a security clearance must have a favorable investigation adjudication by the Department of Defense Central Adjudication Facility reflected properly in the Joint Personnel Adjudication System (JPAS) prior to 1 August 2020. If the CO of the candidate grants an interim clearance, it must be entered in JPAS prior to 1 August 2020. Candidates must maintain security clearance eligibility throughout the advancement cycle to be eligible for advancement.

If, for any reason, a clearance is denied or revoked, an exam is not authorized until a new clearance is granted. PMK-EE is mandatory for cycle 107 candidates and must be completed prior to 1 August 2020. j. Prior service reenlistment (PRISE-R) Sailors required to participate in the NWAE to complete their rate conversion are only required to complete the PMK-EE if they are an E-3 and are eligible for advancement in conjunction with testing to complete rate conversion. k. For guidance on establishing enlisted advancement worksheet (EAW) user roles and completing worksheets, refer to reference (d). EAW is mandatory for cycle 107. ESOs must verify initial TIR eligibility lists on the NEAS website for exam ordering. The deadline for additional orders is 1 July 2020. c. Non-SCI Exams: (1) Initial exam ordering for non-SCI exams will be available until 16 June 2020. ESOs must verify TIR eligibility lists on the NEAS website. (2) Eligible candidates who were not added to the initial TIR list can be added to a supplemental TIR list. Supplemental TIR lists will be available from 17 June 2020 to 19 July 2020. The deadline to order supplemental SELRES exams is 30 September 2020. d. Exams ordered after the TIR lists have closed and exams for prior service reenlistment eligible Reserve Sailors shall be ordered using preferred message formatted e-mail or naval message following the guidelines in chapter 4 of reference (a). Authority is granted for these candidates to be administered exams with the Active-Duty candidates. However, these candidates are still required to take the cycle 107 SELRES exam and not the cycle 248 Active-Duty exam. f. When an exam is not used during the normally scheduled event, do not destroy the exam prior to 1 October 2020. g. Cycle 107 exams should only be administered during the month of September due to coronavirus disease (COVID-19) impact.

(2) To coordinate with COMUSFLTFORCOM and applicable TYCOMs to designate the best-qualified rating SMEs to perform validation and verification of the rating advancement examinations per NETPDTC's examination development and administration schedule (usually the end of March and the end of September). (3) To keep NETPDTC informed of any major changes in the applicable rating and provide an SME to review the advancement exams when a fleet representative has not been identified. What action does NPDC have to take once SMEs are identified.Who announces SME nomination procedures. NETPDTC, in conjunction with NPDC and COMUSFLTFORCOM, will announce nomination procedures by message before each AEDC. What SME selection procedures should be used by major commands. Major commands will use a command nomination process. SME selection should be designed to select the most capable SMEs available. What is the criteria for selecting overseas SMEs. Do not select members from overseas units unless it is determined to be in the best interest of the rating's advancement exam. When should major commands send nominations be received by NETPDTC. No later than 45 days prior to the scheduled AEDC. What must the SME's command be notified about by a major command. Advise the SME's command that NETPDTC will provide accounting data and arrange lodging 2 weeks prior to AEDC commencement. What timelines must the major command comply with. Comply with required timelines specified in the AEDC message. What criteria must direct unit commanders consider when interviewing SME candidates? 1. Hold the rank of chief, senior chief or master chief. Frocked chiefs are discouraged. 2. Possess the skill level for and work in the associated rating. 3. Be familiar with the major types of equipment and duties in their specialty, have extensive background in the rating (including technical training where appropriate courses are available), and have working knowledge of primary reference material in the rating. 4.

Have good communication skills, both in verbal and writing abilities. 5. Have no personal or future TEMADD requirements that would interfere with the availability for the entire AEDC TEMADD, excluding unforeseen emergencies. 6. Have no spouse or immediate family members in the Navy below the rank of chief in the same rating or family member married to someone below the rank of chief in the same rating. How many Chiefs participate in AEDCs. Normally 6 Chiefs Who reviews the rating's advancement exams? An expert from the rating will review the applicable associated series rating exams for content, clarity, flow,correctness and recommend changes to the assigned NETPDTC Team Leader. We'll bring you back here when you are done. Please select the correct language below. Find out how you can intelligently organize your Flashcards. Please upgrade to Cram Premium to create hundreds of folders. Please try again.Please try again.Please choose a different delivery location.It provides instructions for determining eligibility requirements for advancement, preparing necessary forms and messages and effecting advancements and changes in rating. It also contains procedures for ordering, maintaining, and disposing Navy-wide examinations for advancement. If you continue browsing the site, you agree to the use of cookies on this website. See our User Agreement and Privacy Policy.If you continue browsing the site, you agree to the use of cookies on this website. See our Privacy Policy and User Agreement for details.If you wish to opt out, please close your SlideShare account. Learn more. You can change your ad preferences anytime. Why not share! For help you can check writing expert. Check out, please ? www.HelpWriting.net ? I think they are the bestI don't have enough time write it by myself.What is the color for each of the three classifications? Now customize the name of a clipboard to store your clips.

Requests for late exams should be made only for those candidates who, through no fault of their own, are unable to participate on the regularly scheduled NWAE administration date. (1) Since SELRES may take the NWAE anytime in the month of September, Annual Training (AT) and Additional Duty for Training (ADT) orders are not a valid reason for Sailors to take the late exam. Once the Reserve Force ESOs validate the reason of the request, the Command ESO will request approval from NETPDC to allow the Sailor to take the late exam. If exam order discrepancies are noted, contact NETPDC (N321) as listed in the NEAS website at NETPDC (N321) Point of Contact Table. 4. Exam administration and answer sheet returns a. For exam administration and answer sheet return instructions, refer to chapter 6 of reference (a). b. ESOs must check the exam tote board on the NEAS website often to ensure answer sheets have been received and processed. Answer sheets that are in error will not be counted until they are processed successfully. c. A detailed discrepancy list can be found at the NEAS website via MyNavy Portal. For guidance on discrepancy correction requirements, refer to reference (a), chapters 3 and 7. d. Post-exam administration discrepancy correction correspondence with NETPDC (N321) is to be submitted using the EAW post-exam administration comments function, refer to reference (d). Approved late exams must be administered no later than 31 October 2020. 5. Examination results. We appreciate your patience. This manual covers from Beginning Horseman Level I to Advanced Horseman Level IV. Each level has certain requirement a member must meet before they are allowed to proceed to the next level, such as demonstrating basic horse handling and riding, making a feed chart, writing a report, basic horse handling skills and much more. This manual covers from Beginning Horseman Level I to Advanced Horseman Level IV.

Each level has certain requirement a member must meet before they are allowed to proceed to the next level, such as demonstrating basic horse handling and riding, making a feed chart, writing a report, basic horse handling skills and much more. While the CSUSB Visual Identity Manual sets policies and guidelines for using and enhancing CSUSB's visual identity, it also provides users with assistance and flexibility so that the university is visually depicted in a consistent manner. You may also order a printed version from Printing Services. Normal chargeback fees will apply. Personnel meeting minimum time-in-rate (TIR) requirements will be automatically advanced to E2 and E3 without local action. A special performance evaluation is not required to document recommendation for advancement. Additional information for advancement to paygrades E2 and E3 is found in paragraph 704. How long must the Worksheet be kept is a suspense file? ESOs shall hold Worksheets in a suspense file for two years for each respective advancement cycle. E5- 2nd Thursday in March and September. E4- 3rd Thursday in March and September. This PMA will be used solely for the current NWAE and will not be utilized in future PMA computations. When both candidates have finished their respective halves, a proctor will examine each half, remove any markings made by the first user, and exchange the halves. However, the candidates will have no more than 90 minutes to complete their half of the NWAE before the proctor must exchange halves to ensure each candidate is given a fair opportunity to finish the NWAE in the three-hour time period. -Splitting an examination between more than two candidates is not authorized under any circumstances. A command does not need to utilize all quotas in order to request an additional quota (e.g. return an E4 quota and request an E5 quota). Unused MAP quotas are returned to the BSO or major claimant for possible redistribution to subordinate commands.

Do not submit the whole JST only the page showing the degree awarded, institution awarding the degree, and the date awarded. Corrections must be submitted on command letterhead signed by CO or authorized by direction. Letter must contain member’s name, full DoD ID and education points requested. If you notify Harmony of problems with accessing a third-party site that isThis non-discrimination requirement applies to admission to and employment with Harmony Public Schools. Inquiries into issues related to Title IX may be referred to Harmony’s Title IX Coordinator(s) (identified below), to the Assistant Secretary for Civil Rights of the Department of Education, or both. For inquiries regarding non-discrimination policies, pleaseAdmission toLa admision a estos. Please enable it to take advantage of the complete set of features!Get the latest public health information from CDC. Get the latest research from NIH. Find NCBI SARS-CoV-2 literature, sequence, and clinical content:.Secondly, it summarizes the treatment manual of the Tavistock Adult Depression Study, which describes the form of psychoanalytic psychotherapy whose effectiveness has been evaluated both in the Tavistock Adult Depression Study (TADS); and in the German Die Langzeittherapie bei chronischen Depressionen (LAC) Studie. Throughout the history of psychoanalysis, opinions about treatment manuals, empirical research and their antecedents have been deeply divided. After tracing the often polarized unfolding of these matters, the paper proposes that emotional and cognitive difficulties as well as scientific ones underpin their persistence.

It is suggested that greater familiarity with them may lead to better combinations of outcome research and psychoanalysis: for example, the Tavistock manual seeks to match one account of the objects, aims, values, spirit and methods of psychoanalysis (as well as of connected forms of psychoanalytic psychotherapy); and also to meet what is required of treatment manuals by random allocation controlled trials. It has been a crucial element in the above studies of the outcome of long-term psychoanalytic psychotherapies with chronically depressed patients. After describing the Tavistock Manual, the paper concludes suggesting that, if appropriately constructed, treatment manuals can make a contribution to the advancement of specifically psychoanalytic knowledge. It provides essential information about faculty appointments, advancement and promotion, as well as information about faculty life and campus resources. Whether you are considering joining the UCSF faculty or are already a member, we hope that you will find this information useful in all of your academic endeavors. As is often the case with online content, URLs may change from time to time.If your copy of the Faculty Handbook for Success is prior to this date, you should replace it with this version. Some features of WorldCat will not be available.By continuing to use the site, you are agreeing to OCLC’s placement of cookies on your device. Find out more here. Numerous and frequently-updated resource results are available from this WorldCat.org search. OCLC’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus issues in their communities.However, formatting rules can vary widely between applications and fields of interest or study. The specific requirements or preferences of your reviewing publisher, classroom teacher, institution or organization should be applied. Please enter recipient e-mail address(es).

Please re-enter recipient e-mail address(es). Please enter your name. Please enter the subject. Please enter the message. Author: Rebecca Sako; League of Democratic Women (Kaduna, Nigeria). Publisher: Kaduna, Nigeria: League of Democratic Women (LEADS), 1999.Kaduna, Nigeria: League of Democratic Women (LEADS), 1999 (OCoLC)891430257 Please select Ok if you would like to proceed with this request anyway. All rights reserved. You can easily create a free account. IEEE GlobalSpec may share your personal information and website activity with our clients for which you express explicit interest, or with vendors looking to reach people like you. IEEE GlobalSpec will retain this data until you change or delete it, which you may do at any time. You may withdraw your consent at any time.When a webinar seems like a good fit, we will send you an email to invite you to attend. When a white paper seems like a good fit, we will send you an email to invite you to download. Periodically, these affiliates, editorial sponsors, and sponsored content providers will reach out to you via email to determine if you have additional interest in their product or service. Based on information provided at registration (country, job function, industry, etc.), companies may wish to send you email pertaining to their products or service. Please try again in a few minutes.Please try again in a few minutes.Navy, U.S. Navy Reserve, and inactive U.S. Navy Reserve. ThisTo provide procedures for administering the advancement in rate system for enlisted members serving in the U.S. Navy, or U.S. Navy Reserve. This instruction is a complete revision and.To provide procedures for administering the advancement in rate system for enlisted members serving on active duty in the U.S. Navy, U.S. Navy Reserve, and inactive U.S. Navy Reserve. This.Determining advancement eligibility requirements. Preparing necessary forms. Ordering,.

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advansea fx400 manual

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advansea fx400 manual

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advansea fx400 manual

Normally you contac t a marine operator o n their work ing channe l rather th an making initial cont act on C hannel 16. Th e marine oper ator will as k for your intentions and es tablish a paymen t method fo r the call (collect, cre dit card, etc.). When arrangem ents are complete, your ra dio commu nication will be patched into the telephone lin e. In conv ersing with a person on the phone it is important to use normal radio communication procedur es. You should say ”over” and release th e PTT button at the end of each transmission. Both parties can not speak simult aneously as o n normal teleph one calls. Usually t here is a f ee for marine oper ator services which is c harged in addition to any other charge s a ssociated with the call. 3.4 Prohibited Com munication The following co mmunications are prohibit ed b y regulations and violators are subject to penalti es: We're committed to dealing with such abuse according to the laws in your country of residence. When you submit a report, we'll investigate it and take the appropriate action. We'll get back to you only if we require additional details or have more information to share. Note that email addresses and full names are not considered private information. Please mention this; Therefore, avoid filling in personal details. The manual is 3,92 mb in size. If you have not received an email, then probably have entered the wrong email address or your mailbox is too full. In addition, it may be that your ISP may have a maximum size for emails to receive. Check your email Please enter your email address. Post your question here in this forum. We're committed to dealing with such abuse according to the laws in your country of residence. We'll get back to you only if we require additional details or have more information to share. Note that email addresses and full names are not considered private information. Please mention this; Therefore, avoid filling in personal details. Please enter your email address.

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Use of C hannel 16 for calling or hailing must be limited to initial contact only. Calli ng should not exceed 30 seco nds and may b e repeated 3 ti mes at 2 minute intervals. Prior to making contact with anoth er ve ssel, deter mine which cha nnel will be used for continu ed communication after the initial contact. Mon itor the desired channel for traffic and, wh en cl e a r, s w i t c h t o C h a n n e l 1 6 t o m a k e initial contact. Listen for traffi c on the Calling Ch annel (16). If cl ear, pres s the Push To Tal k ( PTT ) button o n the micro phone. Speak the name of the vess el you ar e calling followed by “T his is” and th e name of your vessel an d your call sign. Release the PTT and listen for a reply. When the other vesse l re turns your call, ackn owledge th e call with “go to”, the nu mber of the new cha nnel an d “over”. Switch to the new channel and li st en for traffic. If necessary, wait for traffic to clear, and th en call the o ther ves sel. As commu nication proceeds, end each transmissi on with “over”. When communication with the oth er vessel is compl eted, en d the la st transmission with your call sign and the word “ou t”. It is not necessar y to en d each transmissio n with your call sign, just give your call sign at the beginning a nd end of each c ontact. Remember to sw itch to C hannel 16 when not actively communicating on another channel. 3.3 Telephone Calls You may use your FX-400 transcei ver to make tele phone calls to p ersons on shore. To do so requir es the ser vices of marin e operator s who operate on designat ed Public Corr espondenc e channels. There are seve ral channels designated for this type of traffic and to det ermine t he channel b eing used in you r area, ask som eone wi th lo cal kn owle dge, c onta ct a H arbor M aster or other marine authority. Call the marine operato r and identify yourself with you r vessel’s na me.

User manual FX-400 - 19 -. User manual FX-400 - 20 -. Only numbers are allowed in the ID field. User manual FX-400 - 21 -. User manual FX-400 - 22 -. User manual FX-400 - 23 -. The Data Set menu selections appear in the display. User manual FX-400 - 25 -. User manual FX-400 - 26 -. A warning is displayed after the first attempt and MMSID entry is blocked.User manual FX-400 - 27 -. Menu topics appear in the display. User manual FX-400 - 28 -. The NMEA CHECKSUM menu appears in the display. User manual FX-400 - 29 -. CH NAME Each channel is displayed with default channel name on the second line of the User manual FX-400 - 30 -. User manual FX-400 - 31 -. DSC allows you to contact other DSC equipped vessels and shore stations by selecting their names from your personal call directory, choosing a call type, and pressing a key. DISTRESS! Grounding. HOLD 3 SEC. Select the Nature of Distress User manual FX-400 - 33 -. Re-transmissions of DSC distress calls are canceled automatically when a DSC acknowledgment is received from an authorized SAR coast station, or canceled manually by you. User manual FX-400 - 34 -. Distress sent by another vessel If your FX-400 transceiver intercepts a distress call from another vessel, an alarm sounds and the screen presents time of distress, MMSID of the. User manual FX-400 - 36 -. Except for Distress, each call type is subdivided into one or more priorities. NOTE If the call is to the coast station, you should not select a working channel. Even if you select, FX-400 ignores it when transmits the call.The DSC calling menu appears in the display with the cursor indicating INDIVIDUAL topic. User manual FX-400 - 40 -. The blinking cursor moves to the first digit in the MMSID entry field. User manual FX-400 - 42 -. The Normal Communication screen appears in the display. If there is no response to your call, press the key. The Normal User manual FX-400 - 43 -. The Normal User manual FX-400 - 44 -.

This equipment must be installed and operated in accordance with the instructions contained in this handbook. Introduction Your FX-400 VHF Transceiver is designed for operation in the marine VHF FM frequency band. The operating frequency range is 156.025 to 162.000 MHz which includes all currently allocated International channels. Prior to using your FX-400 inquire with your national radio communication authorities. 2.1 Digital Selective Calling (DSC) Capability You must obtain a nine-digit maritime mobile service identity (MMSI) and program it into the unit before you transmit. Channel 16 must be monitored at all times except when engaged in actual communication on another channel. Channel 16 is monitored by international search and rescue (SAR) authorities, National Coast Guards User manual FX-400 - 8 -. To do so requires the services of marine operators who operate on designated Public Correspondence channels. However, selecting a mounting location affording some protection from the elements will prolong the life of connectors, controls and the liquid crystal display (LCD). Some channels exhibit more background noise than others, so it may be necessary to readjust the squelch setting when changing channels or when scanning. User manual FX-400 - 14 -. Only authorized dealers can program other approved country channels, where specifically allowed by government regulations, by using the 8 pin com connector. Speak directly into the microphone in a normal tone of voice --clearly--distinctly. Say “(name of vessel being called) THIS IS (your vessel’s name and call sign).” User manual FX-400 - 16 -. When scanning is halted by traffic, the scan pauses while the channel is active. User manual FX-400 - 17 -. Memory channels are stored individually and may be deleted individually, or the entire bank may be deleted. If the PTT button is pressed, User manual FX-400 - 18 -. The selected channel is deleted and the channel number is removed from the memory channel bank.

Ihr FX-400 VHF generiert und strahlt elektromagnetische Energie (EME) aus.Die Benutzung von Kanal 16 sollte sich also wirklich nur auf kurze Mitteilungen bzw. Benutzer-Handbuch FX-400 Seite - 8 -. Benutzer-Handbuch FX-400 Seite - 9 -. Benutzer-Handbuch FX-400 Seite - 10 -. Benutzer-Handbuch FX-400 Seite - 11 -. Die LCD-Hintergrundbeleuchtung schaltet sich ein und die Startanzeige wird eingeblendet. Benutzer-Handbuch FX-400 Seite - 13 -. Direkte Anwahl mit den alphanumerischen Tasten am Mikrofon. Benutzer-Handbuch FX-400 Seite - 14 -. Mit den Mikrofontasten werden alphanumerische Zeichen und Symbole wie folgt eingegeben: Benutzer-Handbuch FX-400 Seite - 15 -. Benutzer-Handbuch FX-400 Seite - 16 -. Benutzer-Handbuch FX-400 Seite - 17 -. Benutzer-Handbuch FX-400 Seite - 18 -. Benutzer-Handbuch FX-400 Seite - 20 -. Benutzer-Handbuch FX-400 Seite - 21 -. Benutzer-Handbuch FX-400 Seite - 22 -. Benutzer-Handbuch FX-400 Seite - 23 -. Die Aufschrift LOC erscheint am Ende der Eingabe und weist darauf hin, dass die Ortszeit eingegeben werden muss. Benutzer-Handbuch FX-400 Seite - 24 -. Benutzer-Handbuch FX-400 Seite - 25 -. Benutzer-Handbuch FX-400 Seite - 26 -. Benutzer-Handbuch FX-400 Seite - 27 -. Benutzer-Handbuch FX-400 Seite - 30 -. Benutzer-Handbuch FX-400 Seite - 31 -. Gruppenrufe: nur Routine. Benutzer-Handbuch FX-400 Seite - 32 -. Benutzer-Handbuch FX-400 Seite - 33 -. Benutzer-Handbuch FX-400 Seite - 34 -. Benutzer-Handbuch FX-400 Seite - 35 -. Benutzer-Handbuch FX-400 Seite - 36 -. Die aktuelle Gruppen- MMSI wird in der unteren Zeile angezeigt. Benutzer-Handbuch FX-400 Seite - 37 -. Benutzer-Handbuch FX-400 Seite - 38 -. Benutzer-Handbuch FX-400 Seite - 39 -. Diese Rufliste kann bis zu 32 empfangene Rufe speichern, wobei der zuletzt eingegangene Benutzer-Handbuch FX-400 Seite - 40 -. Benutzer-Handbuch FX-400 Seite - 41 -. Benutzer-Handbuch FX-400 Seite - 42 -. Danach verbleibt die Wechsel-Anzeige, wie im Beispiel oben.Benutzer-Handbuch FX-400 Seite - 44 -.

If you observe the display closely, you will see the channel number change to channel 70 momentarily and then revert to the previous User manual FX-400 - 45 -. Call is made on channel 70. If you observe the display closely, you will see the channel number User manual FX-400 - 46 -. When your FX-400 receives a DSC call from another vessel or a coast station, an alert sounds and a Call Received screen appears in the display. The Call Received screen identifies the time of a call, the caller, the type and the priority of a call. User manual FX-400 - 48 -. All Ship’s calls present Distress, Urgent or Safety information important to all vessels in the area. User manual FX-400 - 49 -. The alert is muted and your transceiver is set to the selected working channel by the caller. 07:16 name or ID GROUP 21 ROUTINE Group Call (Channel changed) User manual FX-400 - 51 -. The Reply screen continues to alternate with the Call Received screen in the display. User manual FX-400 - 52 -. This ATIS ID is derived from your ship call sign. If you are not sure how to derive 10 digits of numeral from your ship call sign, please ask your dealer. User manual FX-400 - 53 -. In this situation, an error beep sounds even if press the PTT key of microphone. TX indication does not appear in the display. User manual FX-400 - 55 -. User manual FX-400 - 56 -. Externally FLASH programmable for various Country Requirements. Vdc Input: 13.6 Vdc. DSC Directory: 32 Entry list with scrolling and Auto Sorting. User manual FX-400 - 58 -. Local Time Offset values can be entered in 30 minute intervals. Instant Access to “Last DSC Call”. User manual FX-400 - 59 -. Image Rejection: Less than -70dB. Intermodulation Spurious Response: Less than -70dB. Noise Level: Less than -40dB unsquelched. User manual FX-400 - 60 -. All vessels equipped with VHF transceivers are required to monitor channel 16. User manual FX-400 - 61 -. User manual FX-400 - 62 -. User manual FX-400 - 63 -.

Voor stationsoproepen worden geen kanalen getoond. User manual FX-400 - 40 -. De knipperende cursor gaat naar het eerste cijfer in het MMSID-invoerveld. Het scherm ALL User manual FX-400 - 42 -. Het menu SET CHannel verschijnt in de display. De huidige groeps-MMSID verschijnt op de display. SET CH 06 08 72 77 GROUP MMSID 087654321 Set Channel User manual FX-400 - 43 -. User manual FX-400 - 44 -. Opmerking: De MMSID’s verschijnen niet in het overzicht. User manual FX-400 - 45 -. Als uw FX-400 een DSC-oproep van een ander vaartuig of kuststation ontvangt klinkt er een signaal en verschijnt het scherm Call Received op de display. Dit scherm toont het tijdstip van de oproep, de oproeper, het type en de prioriteit van de oproep. De meest recente oproep in de oproeplog verschijnt op het scherm. 06:03 name or ID call type 18 priority Controleer het type en de prioriteit van de laatst gelogde oproep. User manual FX-400 - 48 -. User manual FX-400 - 49 -. De noodoproep wordt gedempt en uw marifoon schakelt naar het werkkanaal geselecteerd door de oproeper. User manual FX-400 - 50 -. User manual FX-400 - 51 -. TX op kanaal 06, 08, 10, 11, 12, 13, 14, 15, 17, 71, 72, 74, 75, 76 en 77 hebben ook een beperkt vermogen van 1W. In dit geval klinkt de fouttoon zelfs als u op de PTT toets van de microfoon drukt. Uw FX-400 gaat lang mee, mits u voorzichtig met het toestel omgaat. Neem, om schade te voorkomen, de volgende voorzorgsmaatregelen in acht. User manual FX-400 - 55 -. Extern FLASH programmeerbaar verschillende vereisten Vdc Input: 13.6 V dc. Direct oproepen van willekeurige lognaam Individual Routine Call. Naam oproeper wordt getoond als MMSID in directory staat. DSC-Directory: geheugenplaatsen. Automatische rangschikking scrollfunctie. User manual FX-400 - 57 -. Directe toegang tot “Last DSC Call”. ZENDER Uitgangsvermogen: Instelbaar 25 Watt of 1 Watt.Op duplex kanalen ontvangt uw marifoon op een bepaalde frequentie en zendt op een andere.

Met kuststations kunt u via simplex en duplex kanalen communiceren, maar vanwege het frequentieverschil kunt u niet User manual FX-400 - 59 -. Alle vaartuigen met een VHF marifoon dienen regelmatig kanaal 16 af te luisteren. User manual FX-400 - 60 -. User manual FX-400 - 61 -. User manual FX-400 - 62 -. Its performance can be affected by many factors including equipment failure or defects, environmental conditions, and improper handling or use. It is the user s responsibility to exercise common prudence and navigational judgment, and this device should not be relied upon as a substitute for such prudence and judgment. Your FX-400 VHF radio generates and radiates radio frequency (RF) electromagnetic energy (EME). This equipment must be installed and operated in accordance with the instructions contained in this handbook. Antenna Mounting and EME Exposure For optimal radio performance and minimal human exposure to radio frequency electromagnetic energy, make sure the antenna is: Connected to the radio before transmitting Properly mounted Located where it will be away from people Located at least three feet (91 cm) from the Base Station transceiver and handsets. ELECTRONIC RECYCLING According to the WEEE, this product shall not be treated as household waste. Instead it shall be handed over to the applicable collection point for the recycling of electrical and electronic equipment. For more detailed information about recycling for this product, please contact your local Civic Office, your household waste disposal service or the shop where you purchased the product.It is an advanced marine VHF communication transceiver offering Digital Selective Calling, an easy to use four line LCD display, and a separate Channel 70 receiver. NOTICE Unauthorized changes or modifications to this equipment may void compliance with Regulatory Agency Type Acceptance. Any changes or modification must be approved in writing by the manufacturer.

Benutzer-Handbuch FX-400 Seite - 45 -. DSC-Rufarten: Siehe folgende Tabelle Benutzer-Handbuch FX-400 Seite - 47 -. Benutzer-Handbuch FX-400 Seite - 48. Spiegelselektion: weniger als -70dB. Rauschpegel: weniger als -40dB ohne Sperre.Kanal 16 ist der internationale Not-, Sicherheits- und Rufkanal. Benutzer-Handbuch FX-400 Seite - 50 -. Benutzer-Handbuch FX-400 Seite - 51. Dit is een moderne VHF-marifoon met DSC, een eenvoudig te gebruiken vierregelig LCD-display en een aparte ontvanger voor kanaal 70.Informeer bij uw landelijke overheid op het gebied van radiocommunicatie voordat u uw FX-400 gaat gebruiken. 2.1 Digital Selectieve oproep (DSC) U dient een negencijferig maritiem mobiel service identiteitsnummer (MMSI) te verkrijgen en deze voor het zenden in de unit te programmeren. Kanaal 16 moet te allen tijde beluisterd worden behalve tijdens daadwerkelijke communicatie op een ander kanaal. Kanaal 16 wordt beluisterd door internationale opsporings- en reddingsdiensten (SAR’s) Gebruik van User manual FX-400 - 8 -. Dit vereist de diensten van operators die de aangewezen kanalen voor openbare radiotelefonie bedienen. Er zijn meerdere kanalen aangewezen voor dit type verkeer. De keuze echter van een plaats die bescherming biedt tegen de elementen zal de levensduur van connectoren, bedieningspanelen en het LCD ten goede komen. De meegeleverde datakabel verbindt u de 8-pins connector aan de achterkant van de marifoon en het andere uiteinde sluit u aan op de NMEA data-uitgang van uw GPS-ontvanger. User manual FX-400 - 14 -. Cancelt tevens DSC- en noodoproepen. Kan alleen of met de MEM toets worden gebruikt voor selectie van Priority Scan, Memory Scan of All Scan. User manual FX-400 - 15 -. Het primaire oproepkanaal is 16. Het nummer hiervan verschijnt in de linker bovenhoek van de display. User manual FX-400 - 16 -. Bij onderbreking van het scannen door verkeer, pauzeert Scan User manual FX-400 - 17 -.

Geheugenkanalen worden individueel opgeslagen en kunnen individueel verwijderd worden. Ook kan de gehele bank verwijderd worden. Als de PTT knop wordt ingedrukt, stopt de marifoon met scannen en User manual FX-400 - 18 -. User manual FX-400 - 19 -. User manual FX-400 - 20 -. In de volgende tabel staan de karakters die in het naamveld kunnen worden ingevoerd met behulp van de microfoontoetsen. User manual FX-400 - 21 -. Nadat het laatste MMSID-cijfer is ingevoerd, gaat de cursor terug naar de naamregel op de display. User manual FX-400 - 22 -. User manual FX-400 - 23 -. Het scherm DATA SET verschijnt op de display. User manual FX-400 - 24 -. Het MANUAL LL menu verschijnt op het scherm User manual FX-400 - 25 -. Gebruik een 12 uurs AM-PM User manual FX-400 - 26 -. Het MMSID TYPE menu verschijnt op de display. User manual FX-400 - 27 -. Het TIME FORMAT menu verschijnt op de display.Het CH NAME menu verschijnt op de display. User manual FX-400 - 30 -. User manual FX-400 - 31 -. Met DSC kunt u contact leggen met andere vaartuigen en walstations die met DSC zijn uitgerust, door hun namen te selecteren in uw persoonlijke oproepdirectory, een oproeptype te kiezen en op een toets te drukken. De melding DISTRESS CANCELED verschijnt op de display. User manual FX-400 - 34 -. DISTRESS RELAY VAN EEN ANDER VAARTUIG Als uw FX-400 marifoon een Distress Relay van een ander vaartuig opvangt, klinkt een alarm en verschijnt het volgende scherm op de display. 04:16. Uw FX-400 marifoon verzendt Routine Individual Calls, maar ontvangt zowel Routine als Safety Calls. - Directory Call Directory calls worden gemaakt door de naam van een vaartuig of station te selecteren uit de oproepdirectory. OPMERKING Als u een kuststation oproept, selecteert u geen werkkanaal. Zelfs als u dit doet, zal de FX-400 dit werkkanaal niet meezenden.Op het scherm worden vier toegestane kanalen voorgesteld. U mag ook andere, in uw gebied toegestane kanalen gebruiken.

NOTICE This radio transceiver has been tested and complies with EN v1.3.1 (February 2007). This specification provides reasonable protection against harmful interference in a normal installation. This radio generates, uses and radiates radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to other marine electronic equipment. However, there is no guarantee that interference will not occur in a particular installation. If this radio does cause harmful interference to marine electronic equipment, which can be determined by turning this radio Off and On, the user is encouraged to try to correct the interference by one or more of the following measures: Reorient or relocate the antenna. Increase separation between this radio and other marine electronic equipment. Connect this radio to a power source different from that of other marine electronic equipment. Consult your dealer or an experienced technician for help. Introduction Your FX-400 VHF Transceiver is designed for operation in the marine VHF FM frequency band. The operating frequency range is to MHz which includes all currently allocated International channels. The transceiver has Digital Selective Calling (DSC) capabilities conforming to EN v1.3.1 operation. Distress, All Ships, Individual and Group DSC call formats are supported. There are thirty two memories for storing incoming DSC calls and thirty two for your personal DSC call directory.Prior to using your FX-400 inquire with your national radio communication authorities. 2.1 Digital Selective Calling (DSC) Capability You must obtain a nine-digit maritime mobile service identity (MMSI) and program it into the unit before you transmit. To obtain an MMSI, you will be asked to provide certain information about your ship.

It is important that you obtain an MMSI because National Coast Guards and other search and rescue (SAR) agencies use this information to help speed search and rescue operations. 2.2 Required License Information The following information pertaining to your transceiver is necessary if completing a station license application Output Power. 1 Watt (low) and 25 Watts (high) Emission.16K0F3E, 16K0G3E Frequency Range to MHz Meets Essential Requirements of RTTE DIRECTIVE (Declaration of Conformity) 2.3 Equipment Required The minimum equipment required for two way voice and DSC VHF radio communication with vessels and shore stations includes: VHF radio communication transmitter and receiver designed and approved for marine VHF communication use. VHF antenna and connecting cable. Use a good quality unity gain antenna for best range performance. Power source suitable for the VHF transmitter and receiver. For Digital Selective Calling (DSC) VHF communication radios, connection to a GPS receiver that provides latitude and longitude coordinates and UTC time for distress messages. 2.4 Equipment Supplied FX-400 Marine VHF Transceiver. Microphone with alphanumeric keypad. Mounting Bracket with knobs. Power Cable with in-line fuse (6.3 Amp). NMEA Data Cable. Flush Mounting Kit. Protection cover. User manual FX Sending distress calls automatically uses the Digital Selective Calling (DSC) functions of your transceiver and requires an operating and properly connected navigation receiver. The following procedures are for sending voice distress messages manually. Sending an automatic distress call is described in the DSC section of this manual. 3.1 Using Channel 16 Channel 16 is the Calling and Distress channel. An emergency may be defined as a situation that threatens human life or property. In such situations, make sure your transceiver is turned On and set the channel selector to Channel 16. Then use the following procedure to make a distress call.

The total transmission should not exceed 1 minute. 1. Press the microphone Push To Talk button. Release the Push To Talk button and listen for a reply. 10. If there is no reply, repeat the above message procedure. If there is still no response, try another channel. 3.2 Calling Another Vessel Channel 16 may be used to establish initial contact with another vessel. However, its most important use is for voice emergency messages. Channel 16 must be monitored at all times except when engaged in actual communication on another channel. Channel 16 is monitored by international search and rescue (SAR) authorities, National Coast Guards User manual FX Use of Channel 16 for calling or hailing must be limited to initial contact only. Calling should not exceed 30 seconds and may be repeated 3 times at 2 minute intervals. Prior to making contact with another vessel, determine which channel will be used for continued communication after the initial contact. Monitor the desired channel for traffic and, when clear, switch to Channel 16 to make initial contact. Listen for traffic on the Calling Channel (16). If clear, press the Push To Talk (PTT) button on the microphone. Speak the name of the vessel you are calling followed by This is and the name of your vessel and your call sign. When the other vessel returns your call, acknowledge the call with go to, the number of the new channel and over. Switch to the new channel and listen for traffic. If necessary, wait for traffic to clear, and then call the other vessel. As communication proceeds, end each transmission with over. When communication with the other vessel is completed, end the last transmission with your call sign and the word out. It is not necessary to end each transmission with your call sign, just give your call sign at the beginning and end of each contact. Remember to switch to Channel 16 when not actively communicating on another channel. 3.

3 Telephone Calls You may use your FX-400 transceiver to make telephone calls to persons on shore. To do so requires the services of marine operators who operate on designated Public Correspondence channels. There are several channels designated for this type of traffic and to determine the channel being used in your area, ask someone with local knowledge, contact a Harbor Master or other marine authority. Call the marine operator and identify yourself with your vessel s name. Normally you contact a marine operator on their working channel rather than making initial contact on Channel 16. The marine operator will ask for your intentions and establish a payment method for the call (collect, credit card, etc.). When arrangements are complete, your radio communication will be patched into the telephone line. In conversing with a person on the phone it is important to use normal radio communication procedures. You should say over and release the PTT button at the end of each transmission. Both parties cannot speak simultaneously as on normal telephone calls. Usually there is a fee for marine operator services which is charged in addition to any other charges associated with the call. 3.4 Prohibited Communication The following communications are prohibited by regulations and violators are subject to penalties: User manual FX Messages to any vessel except in emergencies and radio tests. Messages to or from a vessel on land. Transmission while on land. Obscene, indecent, or profane language. 4. INSTALLATION 4.1 Transceiver Your FX-400 Transceiver is designed to withstand the rigors of the marine environment. However, selecting a mounting location affording some protection from the elements will prolong the life of connectors, controls and the liquid crystal display (LCD). Select a location within easy reach and view of the operator and away from your vessel s compass.

Locate the microphone to avoid entanglement with steering or engine controls, both when in use and when stowed. Also, consider routing of antenna, power and NMEA interface cables. Mount the transceiver securely to a solid surface. 4.2 Antenna Proper installation of a quality VHF antenna is very important to reliable radio communication. A good quality unity gain antenna is recommended for maximum range performance. In general, antennas should be located as high as practical and separated as much as possible from other antennas and structures. The minimum distance to other objects is 1 meter. Route the antenna cable away from other electronic equipment and do not bundle the antenna or power cable with other wiring, especially transducer cables for depth sounders and fish finders. Mount the antenna and install the connector(s) in accordance with manufacturer s instructions. Connect the antenna cable to the RF output connector on the rear panel of the transceiver. 4.3 Power Connection CAUTION Reverse polarity connections can damage your transceiver The power cable for you transceiver must be connected to the ships main power buss. Use the 6.3 Amp in-line fuse provided. Connect the barrel terminals on the power cable to the matching color wires and terminals extending from the rear panel of the transceiver. User manual FX. User manual FX Firmly attach the bolt foot rotating to the top of the screw. (See Fig. 1) 4. Firmly fix the plastic mount block on the either side of the radio using shorter screw. Don t forget to attach the washer. (See Fig. 2 and Fig. 3) 5. Fasten the longer screw to fix the radio to the dash board firmly. (Fig. 4) The same works should be done to the other side too.The 4-line LCD displays the current operating status, menus for selecting functions, and settings for optional features. The microphone has a keypad for changing channels and selecting functions. When the VOL knob is in the full CCW position the unit is turned Off.

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